Amino acid:tRNA ligases (EC 6.1.1.-)

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Characterization of Amino Acid tRNA Ligases using the Analytical Ultracentrifuge

Quaternary structures of amino acid tRNA ligases/synthetases (aaRS) in the native as well as in denatured forms were examined by molecular weight determinations (paper I-IV). Analytical ultracentrifugation, gel electrophoresis, gel chromatography were used in these investigations. The aaRSs were obtained from bacteria and yeast: LysRS and ValRS from S. cerevisiae, AspRS, LysRS and SerRS from E....

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[Novel L-amino acid ligases catalyzing oligopeptide synthesis].

L-Amino acid ligase (EC 6.3.2.28) is a microbial enzyme catalyzing formation of an alpha-peptide bond from unprotected L-amino acids in an ATP-dependent manner. The YwfE protein from Bacillus subtilis 168 was the first reported L-amino acid ligase, and it synthesizes various dipeptides. Thereafter, several L-amino acid ligases were newly obtained by in silico analysis using the ATP-grasp motif....

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Mechanism of action of amino acid transfer ribonucleic acid ligases.

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ژورنال

عنوان ژورنال: FEBS Letters

سال: 1980

ISSN: 0014-5793

DOI: 10.1016/0014-5793(80)80253-5